Surface Loop Motion in FepA

Surface loop motion in FepA.

Using a lysine-specific cleavable cross-linking reagent ethylene glycolbis(sulfosuccimidylsuccinate) (Sulfo-EGS), we studied conformational motion in the surface loops of Escherichia coli FepA during its transport of the siderophore ferric enterobactin. Site-directed mutagenesis determined that Sulfo-EGS reacted with two lysines, K332 and K483, and at least two other unidentified Lys residues i...

Concerted loop motion triggers induced fit of FepA to ferric enterobactin

Spectroscopic analyses of fluorophore-labeled Escherichia coli FepA described dynamic actions of its surface loops during binding and transport of ferric enterobactin (FeEnt). When FeEnt bound to fluoresceinated FepA, in living cells or outer membrane fragments, quenching of fluorophore emissions reflected conformational motion of the external vestibular loops. We reacted Cys sulfhydryls in sev...

The Journal of General Physiology Concerted loop motion triggers induced fit of FepA to ferric enterobactin

microleakage evaluation in regard to cavo-surface designs & treatments

چکیده ندارد.

Loop Quasi-Invariance Code Motion

Loop optimization plays an important role in compiler optimization and program transformation. Many sophisticated techniques such as loop-invariance code motion, loop restructuring and loop fusion have been developed. This paper introduces a novel technique called loop quasi-invariance code motion. It is a generalization of standard loop-invariance code motion, but based on loop quasi-invarianc...